| Km |
|
Bacterial kanamycin resistance gene (also called kanR). |
| Cm |
|
Bacterial chloramphenicol resistance gene. |
| Hyg |
|
Plant hygromycin resistance gene. |
| Bar |
|
Gene encoding resistance to the herbicide BASTA. |
| OCS 3' |
|
Poly adenylation signal sequence from Agrobacterium tumefaciens. |
| MAS 3' |
|
Poly adenylation signal sequence from Agrobacterium tumefaciens. |
| MAS 1' |
|
Plant promoter from Agrobacterium tumefaciens . |
| CaMV 34S |
|
Viral promoter; our constructs have the omega leader of TMV (Gene 1987;60(2-3):217-25 ) at the end of the promoter sequence; this sequence is thought to increase translation efficiency, but it is not expected to influence RNAi. |
| LB |
|
T-DNA left border. |
| RB |
|
T-DNA right border. |
| GUS |
|
360 base pair fragment from the GUS (Escherichia coli beta-glucuronidase gene). |
| ChsA intron |
|
1,353 bp fragment from the petunia Chalcone synthase A gene. |